Advanced search
Advanced search
Advanced search
Advanced search
Advanced search
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-p-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with; 1/1, 2/2, 5/5, 10/10 (lmg/dm31 2,4-D/lmg/dm3] 6-BAP) and 1/1/1,2/2/2, 5/5/5, 10/10/10 ([mg/dm31 2,4-D/(pM/dm3j 17-p-estradiol/(mg/dm3) 6-BAP) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.
Biotechnologia, vol.65, 2 (2004)-.
0860-7796 ; oai:rcin.org.pl:122362
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Apr 29, 2020
272
https://rcin.org.pl./publication/152733
Edition name | Date |
---|---|
Densytometryczna analiza diosgeniny w ekstraktach z tkanki kalusowej Polygonatum verticillatum (L.) Ali. | Oct 2, 2020 |
Szybka-Hrynkiewicz, Przemysław Janeczko, Zbigniew
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.