Subtitle:

Micropropagation of Miscanthus x giganteus (Greef & Deu.) from inflorescence explants

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

2 mm explants of immature inflorescences (0,5-3 cm) produced calluses on MS medium with 0,5 mg x M BAP and 2,5 mg x M 2,4-D. The surface sterilization in 10% sodium hypochloride for 20 min was successful in 74%. After 20 days of incubation, the surfaces of all explants were covered by callus. After 2 months of culture, from most of calluses embryo-like structures developed. The highest frequency of fully regenerated plants was observed on MS medium supplemented with 1-2 mg X |-> BAP. The regeneration rate of the cultured calluses for the first two weeks in 16 h day fotoperiod was twice as high as the one under a continuous light. Only two out of 6 thousand cultured mature spikelets produced calluses from which 574 plants were obtained after multiplication. Most of the plants obtained from the explants representing immature as mature inflorescences survived the transfer into soil in the greenhouses.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

251

End page:

259

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122765

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open