Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
One of the possibilities of the conservation biology is the in vitro culture method. The usefulness of in vitro culture methods of spore planting and prothallium storage of rare, threatened and protected fern species were tested. Spors of Asplenium adulterinum, A. cuneifolium, A. septentrionale and Polypodium vulgare were collected from sporophytes growing in the natural habitats of Lower Silesia area in 1996. The collected spores were disinfected and the aseptic spores were cultured in flasks containing 1/4 MS solid medium. After three to eight months, the spores germinated, giving rise to a filamentous gametophytes. Six to eight months later, spotophytes could be observed in these cultures. The in vitro method gives the possibility to investigate the special characteristics of life cycle and breeding system of fern species. It is possible to collect fern spores from natural habitats and use them to obtain populations, which will allow to preserve the fern population in cultivation in the Botanical Garden of the Wroclaw University.
Biotechnologia, vol.65, 2 (2004)-.
0860-7796 ; oai:rcin.org.pl:122762
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
2 paź 2020
30 kwi 2020
635
https://rcin.org.pl./publication/153193
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Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
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Nowak, Jacek K.