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Lisek, Anna ; Korbin, Małgorzata ; Żurawicz, Edward ; Rozpara, Elżbieta
Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD-PCR technique
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except ‘Węgierka Zwykła’ and clones ‘Promis’, ‘Tolar’, ‘Nectavit’.
Biotechnologia, vol.65, 2 (2004)-.
application/pdf ; application/pdf
0860-7796 ; oai:rcin.org.pl:122353
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 29, 2020
75
https://rcin.org.pl./publication/152718
Edition name | Date |
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Analiza polimorfizmu DNA odmian śliwy (Prunus domestica L.) przy użyciu techniki RAPD-PCR | Oct 2, 2020 |
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