Piątczak, Ewelina ; Wielanek, Marzena ; Królicka, Aleksandra ; Łojkowska, Ewa ; Wysokińska, Halina
Hairy root culture of Centaurium erythraea Rafn
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves with agropine strain of Agrobacterium rhizogenes (LBA 9402). The frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As a result of transformation, 30 clones of hairy roots were obtained. These clones were grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times, and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentio- picroside, swertiamarine).
Biotechnologia, vol.65, 2 (2004)-.
application/pdf ; application/pdf
0860-7796 ; oai:rcin.org.pl:122343
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 29, 2020
532
https://rcin.org.pl./publication/152701
Edition name | Date |
---|---|
Kultury korzeni transformowanych Centaurmm erythraea Rafn | Oct 2, 2020 |
Królicka, Aleksandra Łojkowska, Ewa
Królicka, Aleksandra Kurlenda, Julianna Łojkowska, Ewa
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Wielanek, Marzena Urbanek, Henryk
Wysokińska, Halina