Metadata language
Szybka-Hrynkiewicz, Przemysław ; Janeczko, Zbigniew
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-p-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with; 1/1, 2/2, 5/5, 10/10 (lmg/dm31 2,4-D/lmg/dm3] 6-BAP) and 1/1/1,2/2/2, 5/5/5, 10/10/10 ([mg/dm31 2,4-D/(pM/dm3j 17-p-estradiol/(mg/dm3) 6-BAP) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.
Relation:Biotechnologia, vol.65, 2 (2004)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format:application/pdf ; application/pdf
Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
Projects co-financed by: Access: