Subtitle:

Densitometric analysis of diosgenin in extracts from callus tissue of Polygonatum verticillatum (L.) All.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In this study, we examined the effect of plant growth regulators (2, 4-D, 6-BAP) and steroidal hormone (17-p-estradiol) on a quantity of diosgenin in callus tissue of Polygonatum verticillatum (L.) All. The callus grown in in vitro conditions on MS medium with; 1/1, 2/2, 5/5, 10/10 (lmg/dm31 2,4-D/lmg/dm3] 6-BAP) and 1/1/1,2/2/2, 5/5/5, 10/10/10 ([mg/dm31 2,4-D/(pM/dm3j 17-p-estradiol/(mg/dm3) 6-BAP) was used for the densitometric analysis to determine the presence of diosgenin. The content of diosgenin ranged from 0,0642 to 1,1458%. An increase in diosgenin content due to addition of 6-BAP and 2,4-D was observed. The developed procedure was estimated by statistical analysis which indicates that it can be used for routine analysis.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

93

End page:

99

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122362

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open