Subtitle:

Induction, proliferation and fitochemical analysis of callus tis»ue of Polygonatum verticillatum (L.) All.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In this study, we examined the effect of; coconut water (CW), plant growth regulators NAA - a-naphthaleneacetic acid, IBA - indole-3-butyric acid, 2,4-D-2,4-dichlorophenoxyacetic acid as well as 6-BAP - 6-benzyl amintpurine and steroidal hormones (progesterone, estriol, estrone, 17-p-estradiol, andro.terone, androstendione) on callus of Polygonatum verticillatum (L.) All. induction The best callus formation was obtained from the hypocotyl part ol plants cultivated from the seeds after their stratification (two weeks in 4°C)on the Murashige and Skoog (MS) medium, which contained: 100 mg/cm^ of myoinositol, 30 g/dm^ of sucrose, 2 mg/dm^ of 2,4-D and 1 pM/lm^ of 17-p-estradiol. Additionally, the efforts to obtain the callus from underground parts of P. verticillatum were made. It was done on MS medium conaining: 1 mg/dm^ 2,4-D, 1 pM/dm^ 17-p-estradiol and 2 ing/dm^O - BAP. The callu; grown in in vitro conditions was used for the fitochemical analysis to determne the presence of diosgenin.

Relation:

Biotechnologia, vol.66, 3 (2004)-.

Volume:

66

Issue:

3

Start page:

254

End page:

259

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:119512

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open