Subtitle:

Celi suspension obtained from callus or directly from cotyledons of Pharbitis nil Chois

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

An attempt has been made to obtain cell suspension from callus as well as directly from cotyledons P. nil. Cotyledons of 7-days old sterile seedlings and flower buds excised from 3-week old plants were the material for the induction of callus. The explants were laid out on MS medium supplemented with various combination of plant hormones; BAP (5 mg/1) and NAA (1 mg/1) and supplemented with 3% sucrose or 2% glucose and \% sucrose, BAP (5 mg/I) and I/\A (0,5 mg/I), BAP (0,5 mg/1) and Picloram (1 mg/I), BAP (5 mg/1) and Picloram (0,5 mg/1), 2,4-D (0,125 mg/1). The cultures were grown in continuous white light or in darkness. The callus obtained from cotyledons cultivated in darkness and callus from flower buds cultivated in light on MS medium with BAP (5 mg/1), N/\A (I mg/1), 2% glucose and ]% sucrose were proved useful for obtaining cell suspension. Moreover, an attempt was made to obtain cell suspension directly from cotyledons. Cotyledons were cut into small fragments or were subjected to enzymatic digestion. The cell suspensions were cultivated on MS medium with the addition of BAP (5 mg/1) and l/\A (0,5 mg/I) on a shaker at 140 rpm. The increase of cell number was determined by counting the cells every 5 days. In the subsequent subcultures, a decrease of the number of cell divisions was observed.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

219

End page:

224

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122755

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open