Inny tytuł:

The effect of cytokinins on morphogenetic response of Polemonium coeruleum seedlings explants

Współtwórca:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Wydawca:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Opis:

prace eksperymentalne

Typ obiektu:

Journal/Article

Abstrakt:

The aim of the presented research was to examine the morphogenetic response of Polemonium coeruleum explants. The donor material were 10-day-old seedlings. Surface sterilized seeds were germinated on MS medium supplemented with CA3 (1 mg dnr^). Seedling explants (shoot tips, fragments of cotyledons, hipocotyls and roots) were isolated and transferred onto solidified MS medium supplemented with different types of cytokinins (BA, KN, ZEA, 2iP) at concentrations 1.0, 3.0 and 5.0 mg dm ^ in combination with NAA (0.1 mg dnr^). All explant types were characterized by callus proliferation. It was observed that calli developed on the entire surface of hipocotyl and root fragments. On the other hand, shoot tips and cotyledonary petioles formed callus tissue at the cut ends, and petioles only at abaxial ends. The growth of calli on all explant types was strongly stimulated by ZEA. Among the explants tested, only shoot tips exhibited shoot organogenesis. The highest frequency of shoot organogenesis was observed when the explants were cultured on a medium supplemented with 5.0 mg dm-3 BA (lOO^o) or 5.0 mg dnr^ ZEA (97%). The highest shoot number per explant was obtained in the presence of 5.0 mg dnr^ZEA (8.4 on average). The presence of BA or ZEA in the proliferation medium inhibited rhizogenesis and the elongation growth of shoots. However, root organogenesis was supported by KN added into the medium.

Czasopismo/Seria/cykl:

Biotechnologia, vol.89, 2 (2010)-.

Tom:

89

Zeszyt:

2

Strona pocz.:

125

Strona końc.:

131

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

oai:rcin.org.pl:71607 ; IChB B-84

Źródło:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open