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Micropropagation of Salvia officinalis L. by shoot tips
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 pmol/1 lAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 pmol/1 was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was 1/2 MS agar medium without growth regulators.
Biotechnologia, vol.65, 2 (2004)-.
0860-7796 ; oai:rcin.org.pl:122751
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Apr 30, 2020
469
https://rcin.org.pl./publication/153182
Edition name | Date |
---|---|
Mikrorozmnażanie Salvia officinalis L. z wierzchołków pędów | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Wysokińska, Halina
Wysokińska, Halina
Nowak, Jacek K.