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Micropropagation of Salvia officinalis L. by shoot tips
Subtitle:Micropropagation of Salvia officinalis L. by shoot tips
Creator:Grzegorczyk, Izabela ; Wysokińska, Halina
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:In this study, optimum conditions for the micropropagation of Salvia officinalis by shoot tips were determined. Shoot tips from five-week-old shoots grown in in vitro culture were used as explants. The explants were incubated on MS agar medium supplemented with 0.57 pmol/1 lAA and various concentrations of cytokinins (BAP, zeatin, TDZ or kinetin). The best results were obtained when BAP at the concentration of 2 pmol/1 was used as cytokinin. Under these conditions after 5 weeks more than 90% shoot tips formed axillary buds or shoots and almost 3 shoots per one explant were obtained. An average length of shoots was 2.5 cm. For root induction the most suitable was 1/2 MS agar medium without growth regulators.
Relation:Biotechnologia, vol.65, 2 (2004)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format:application/pdf ; application/pdf
Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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