Subtitle:

In vitro organogenesis induction in Sida hermaphrodita Rusby

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Sida hermaphrodita Rusby belongs to the Malvaceae family. It is a perennial plant. Biomass obtained from sida plantations may constitute excellent raw material for renewable energy production. Sida hermaphrodita Rusby reproduces generatively, but its vegetative reproduction is also possible by root-cuttings, division of the underground part into particular roots, or cuttings obtained from green above-ground shoots. Sida seeds should be sown not earlier than after one year of storage, because immediately after harvest the percentage of germinated seeds is as low as 2%, after twelve months of storage 77.5%, to drop in successive months. Moreover, due to their hardness, sida seeds require special treatment. Therefore, an attempt was made at developing a method for in vitro micropropagation of sida plants, as an alternative method of seed material production. Two kinds of explants were used in the experiment, i.e. leaf discs 8 mm in diameter and 3 mm fragments of leaf stalks. The growth regulators were added to the MS medium in combinations, i.e.: lAA -I- kinetin; lAA -P kinetin + BAP; TDZ -I- 2.4-D; 2ip + 2.4-D. The callus formed was crumbled and transferred to two kinds of medium: MS without growth regulators and MS with kinetin, NAA, GA3 and 40 g H of sucrose. The most intensive growth of callus was observed on media containing 2ip and 2,4-D, but the highest number of roots and developing shoots was obtained on MS medium without fitohor- mones.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

131

End page:

136

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122377

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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