Screening of Polish red clover cultivars for their capability to somatic embryogenesis
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Ten Polish red clover cultivars were tested in vitro for their capability to produce callus and somatic embryos. A three-step tissue culture protocol (callus induction, embryo induction and plant development) based on Gamborg’s B5 basal salts, NAA and 2,4-D as auxins, and kinetin and adenine as cytokinins was utilized. Explants source were petiole and hypocotyl sections. All clover varieties under study have showed high efficiency of callus production, and low ability for plant regeneration from the callus. The highest percent of genotypes which developed embryos was observed on variety Parka (3,6). Single embryogenic genotypes were selected from nearly all varieties. Hypocotyls showed the greatest ability for callus regeneration comparing to leaf petioles.
Biotechnologia, vol.65, 2 (2004)-.
application/pdf ; application/pdf
0860-7796 ; oai:rcin.org.pl:122373
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 29, 2020
230
https://rcin.org.pl./publication/152755
Edition name | Date |
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Ocena polskich odmian koniczyny czerwonej pod względem zdolności do somatycznej embriogenezy | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan