Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The aim of this investigation was to establish the conditions for effective regeneration of adventitious shoots on leaf explants of apple dwarf rootstock P.59. A three stage procedure was used - the induction on medium containing thidiazuron (TDZ) and a-naphtalene acetic acid (NAA) for two weeks, then the regeneration on medium supplemented with 6-benzylaminopurine (BAP) and NAA and growth of adventitious shoots on medium with low concentration of BAP and indole-3-butyric acid (IBA). The youngest leaves from proliferating shoot cultures were taken as explants for adventitious regeneration. The effect of MS salt concentration and type of solidifying agent in the induction and regeneration media, and a type of cytokinin in the induction medium was studied. It was proved that the regeneration potential was higher when 1/2 MS salts, Gelrite and TDZ was used.
Biotechnologia, vol.65, 2 (2004)-.
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Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Apr 30, 2020
192
https://rcin.org.pl./publication/153167
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