Subtitle:

Effect of bulb chilling and growth regulators on the in vitro bulblet regeneration in Hippeastrum x chmielii Chm.

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Description:

prace eksperymentalne

Abstract:

Selected clones of a new intergeneric species Hippeastrum x chmielii Chm. grow vigorously, flower abundantly and produce flowers in clear, vivid colors. The hybrid is suitable both for cut flower production and as a pot plant. A great demand for planting the material is expected, therefore it is necessary to elaborate an efllcient micropropagation method where due to application of growth regulators the bulblets can be differentiated from different tissues. To meet this goal, the trials were undertaken to propagate this hybrid from inflorescence shoots (scapes) obtained from bulbs (clone 2/7) chilled for 3, 5 or 7 months at 4°C. Both the length of chilling and the growth regulators in the MS medium affected bulblet regeneration. On the medium without growth regulators bulblets were scarce and appeared only on shoot explants from bulbs chilled for 5 months. The presence of 2 mg dm-^ isopenthenyladenine (2iP) and 0,2 mg dm'^ a-naphtylacetic acid (NAA), as well as combination of the same auxin with 2 mg dm-3 benzyladenine (BA), stimulated bulblet formation on the explants. After 3 months of chilling, the regeneration was the poorest and the longer the chilling period was the more bulblets were produced on explants, but only in the presence of growth regulators.

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

185

End page:

190

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122724

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open