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Obtaining double haploid transgenic tobacco lines by the tissue culture method
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
In the study on double haploid (DH) lines of tobacco, two cultivars - MN944 and BY103 transformed with a construct carrying the coat protein gene of Lettuce Mosaic Virus (LMV) CP were used. The hybrids F] from crossing transgenic line BY 103 with its non-transformed equivalent as well as transgenic MN944 line with cv. Wiślica were used for producing haploids via inducted androgenesis. Double haploids were received by regeneration from stem fragments. The course of meiosis in pollen mother cells was analysed and pollen viability was estimated.
Biotechnologia, vol.65, 2 (2004)-.
0860-7796 ; oai:rcin.org.pl:122344
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Apr 29, 2020
263
https://rcin.org.pl./publication/152702
Edition name | Date |
---|---|
Uzyskiwanie transgenicznych linii podwojonych haploidów tytoniu metodą kultur tkankowych | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Doroszewska, Teresa Chachulska, Anna Maria
Nowak, Jacek K.