Subtitle:

Hairy root culture of Centaurium erythraea Rafn

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves with agropine strain of Agrobacterium rhizogenes (LBA 9402). The frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As a result of transformation, 30 clones of hairy roots were obtained. These clones were grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times, and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentio- picroside, swertiamarine).

Relation:

Biotechnologia, vol.65, 2 (2004)-.

Volume:

65

Issue:

2

Start page:

29

End page:

36

Detailed Resource Type:

Article

Format:

application/pdf ; application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:122343

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open