Inny tytuł:

Hairy root culture of Centaurium erythraea Rafn

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves with agropine strain of Agrobacterium rhizogenes (LBA 9402). The frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As a result of transformation, 30 clones of hairy roots were obtained. These clones were grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times, and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentio- picroside, swertiamarine).

Czasopismo/Seria/cykl:

Biotechnologia, vol.65, 2 (2004)-.

Tom:

65

Zeszyt:

2

Strona pocz.:

29

Strona końc.:

36

Szczegółowy typ zasobu:

Article

Format:

application/pdf ; application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:122343

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open