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RCIN and OZwRCIN projects

Object

Title: Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Subtitle:

Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.

Relation:

Biotechnologia, vol.44, 1 (1999)-.

Volume:

44

Issue:

1

Start page:

164

End page:

171

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:143459 ; IChB B-40

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty

Object collections:

Last modified:

Nov 18, 2020

In our library since:

Oct 19, 2020

Number of object content downloads / hits:

46

All available object's versions:

https://rcin.org.pl./publication/178653

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