Starzycki, Michał ; Starzycka, Eligia ; Matuszczak, Marcin
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.
Biotechnologia, vol.44, 1 (1999)-.
0860-7796 ; oai:rcin.org.pl:143459 ; IChB B-40
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 18, 2020
Oct 19, 2020
44
https://rcin.org.pl./publication/178653
Edition name | Date |
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Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens | Nov 18, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan