Title:

Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Subtitle:

Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Creator:

Starzycki, Michał ; Starzycka, Eligia ; Matuszczak, Marcin

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1999

Subject and Keywords:

biotechnology

Abstract:

The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.

Relation:

Biotechnologia, vol.44, 1 (1999)-.

Volume:

44

Issue:

1

Start page:

164

End page:

171

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-40

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open