Tytuł:

Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Inny tytuł:

Regeneration of winter oiiseed rape and trials of transformation mediated by Agrobacterium tumefaciens

Twórca:

Starzycki, Michał ; Starzycka, Eligia ; Matuszczak, Marcin

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Data wydania/powstania:

1999

Temat i słowa kluczowe:

biotechnology

Abstrakt:

The effects of one strain Agrobacterium tumefaciens LBA 4404/pBl 121, conditions of cocultivation and growth regulators on regeneration were studied and trials of transformation wereperformed on winter oilseed rape Brassica napus L. cv. Lirajet, Valesca and Bor. Strong shootsregeneration was observed after co-cultivation without acetosyringone. Among antibiotics thatwere used in the experiments, kanamycin A and geneticin hampered whereas carbenicillin andrifampicin stimulated shoots regeneration. The presence of npt 11 gene was confirmed by PCRand Kl, K2 primers which generate a fragment of 732 bp in length.

Czasopismo/Seria/cykl:

Biotechnologia, vol.44, 1 (1999)-.

Tom:

44

Zeszyt:

1

Strona pocz.:

164

Strona końc.:

171

Typ zasobu:

Text

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; IChB B-40

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

eng

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open