Subtitle:

Mutageneza mysich pierwotnych komórek zarodkowych (Embryonic Stem Cells)

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Embryonic stem (ES) cells derived from preimplantation mouse embryo provide a powerfultool for genome manipulation in mammals. The two principal genetic approaches are used tomodify genomes of embryonic stem cells, which may be introduced into blastocyst to producechimeras, and these animals transmit the genetic alteration into the next generation. One approach, targeted mutagenesis, is designed to disrupt the function of specific murine genes thatare known by their homology to genes of other organisms. The other approach, gene trappingby randomly insertional mutagenesis, is designed to identify novel, developmentally regulatedgenes in mouse embryos. In vivo screens allow for the identification and studying of genes thatare expressed either within specific tissue or in spatiotemporal patterns. As an alternative to invivo gene study, gene expression within specific cell types may be monitored in different ES cellcultures.

Relation:

Biotechnologia, vol.44, 1 (1999)-.

Volume:

44

Issue:

1

Start page:

94

End page:

105

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:143453 ; IChB B-40

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open