Subtitle:

Nucleic acid in sifu hybridization; from ISH to DIRVISH

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In situ hybridization (ISH) provides a highly sensitive method for the detection of specificnucleic acid sequences in cells, nuclei and metaphase chromosomes. This technique has beenimproved continuously since it was first established in 1969. Especially fluorescent in situ hybridization (FISH) rapidly replaced radioactive procedures and became a conventional tool incytogenetic research. ISH plays an increasingly important role in a variety of research areas ofmedicine, genetics and plant breeding. It has been successfully applied for chromosome orchromosome fragments identification, chromosomal abnormalities detection, gene mapping orspecific DNA sequences localization.

Relation:

Biotechnologia, vol.29, 2 (1995)-.

Volume:

29

Issue:

2

Start page:

92

End page:

101

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:147872 ; IChB B-25

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open