Sposoby identyfikacji markerów RAPD w rośiinach
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The random amplified polymorphic DNA (RAPD) method is based on random amplificationof DNA fragments, via PCR, using short primers of arbitrary sequence. RAPD markers have beenapplied to construct linkage maps, to assess genetic diversity, to study taxonomic relationships,and to tag disease resistance genes in plants. RAPD markers linked to a resistance gene canbe identified using bulked segregant analysis (BSA), recombinant inbred lines (RlLs) or nearisogenic lines (NlLs). More reliable and specific PCR-based markers known as sequence-characterized amplified region (SCAR) and allele-specific associated primer (ASAP) were developed. Thereare several examples of the application of these DNA marker systems in marker-assisted plantbreeding.
Biotechnologia, vol.44, 1 (1999)-.
0860-7796 ; oai:rcin.org.pl:143454 ; IChB B-40
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Nov 18, 2020
Oct 19, 2020
766
https://rcin.org.pl./publication/178646
Edition name | Date |
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Sposoby identyfikacji markerów RAPD w rośiinach | Nov 18, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan