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Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
An approach for enzymatic synthesis of oligosaccharides via transglycosylation with endo-p1,3-glucanase from Oerskovia xanthineolytica is described. Linear -p-l,3-glucan (laminarin) wasused as the donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors. The synthesis was performed in water-organic solvent environmentwith several combinations of donor/acceptor. Employing p-nitrophenyl-P-D-xylopyranoside as anacceptor in the presence of 30% acetonitrile resulted in the production of six new glycosidesshown by FAB MS to be di-, tri- and tetrasaccharides. This enzyme is therefore suitable for thesynthesis of short-chain oligosaccharides.
Biotechnologia, vol.33, 2 (1996)-.
0860-7796 ; oai:rcin.org.pl:147112 ; IChB B-29
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
13 lis 2020
13 lis 2020
57
https://rcin.org.pl./publication/182766
Nazwa wydania | Data |
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Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica | 13 lis 2020 |
Buchowiecka, Alicja Bielecki, Stanisław
Buchowiecka, Alicja Bielecki, Stanisław
Bielecki, Stanisław Buchowiecka, Alicja Gajewska, Małgorzata
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan