Title:

Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica

Subtitle:

Short-chain oligosaccharide synthesis by endo-B-1,3 glucanase from Oerskovia xanthineolytica

Creator:

Bielecki, Stanisław ; Buchowiecka, Alicja

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Date issued/created:

1996

Subject and Keywords:

biotechnology

Abstract:

An approach for enzymatic synthesis of oligosaccharides via transglycosylation with endo-p1,3-glucanase from Oerskovia xanthineolytica is described. Linear -p-l,3-glucan (laminarin) wasused as the donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors. The synthesis was performed in water-organic solvent environmentwith several combinations of donor/acceptor. Employing p-nitrophenyl-P-D-xylopyranoside as anacceptor in the presence of 30% acetonitrile resulted in the production of six new glycosidesshown by FAB MS to be di-, tri- and tetrasaccharides. This enzyme is therefore suitable for thesynthesis of short-chain oligosaccharides.

Relation:

Biotechnologia, vol.33, 2 (1996)-.

Volume:

33

Issue:

2

Start page:

184

End page:

191

Resource type:

Text

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; IChB B-29

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open