Subtitle:

Early stages of embryogenesis after fertilization in in vitro condition

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Ovules of plum cultivars (Sweet Common Prune, Herman, Gilbert, SanctusHubertus, Cacanska Rodna, Ćaćanska Najbolia) were pollinated with pollen ofcv. Stanley on the White medium with addition of 15% sucrose. The nucellusesexcised from the fertilized ovules were cultivated on Norstog medium with addition of 3,4% sucrose. The process of fertilization after pollination in the in vitro condition was determined on the basis of the paraffin cross-sections: thedegeneration of synergid after entering the pollen tube, the fertilization of eggcell were confirmed. The microscopic observations showed the swelling ofmicropylar part of nucellus. The paraffin cross-sections showed a many-layerkalotka (peculiar morphological structure) and swelled cells of nucellus amongwhich the embryo sac was observed. The presence of embryo without endosperm or the presence of endosperm without embryo in the embryo sac wasnoted.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

54

End page:

56

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139250 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open