Subtitle:

Possibility of the application of various fragments of maize (Zeamays L.) embryos in cultures in vitro

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The aim ofthe performed investigations was to determine which part oftheembryo has the optimal properties for callus induction at low concentrations of2,4-D (2 mg dm-3).The explants were cut along or across the embryo axis into 2, 3, 4 fragments and cultured on the Murashige and Skoog medium for 6 weeks. It wasfound that callus derived from mature embryos (dry seeds) is characterised bycomparable fresh and dry mass and by similar changes in size of the cells withfragments derived from mature embryos (but before the dormant) and from immature embryos. Essential difference was observed in the germination ability ofthe explants. The percentage of germination of mature embryo fragments wasthe lowest. It was demonstrated that despite of the embryo type, fragmentsoriginating from its central part appeared the most suitable for in vitro culture.In the case of these fragments the highest increase of the fresh mass of callustissue, optimal morphological features and the appearance of meristematic centres were observed.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

111

End page:

117

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139316 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open