Subtitle:

Regeneration of Arabidopsis thaliana (L.) Heynh. plants via directsomatic embryogenesis

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

In Arabidopsis biotechnology plants are regenerated in vitro via shoot organogenesis induced in callus derived from different somatic tissues. An alternative way of in vitro plant regeneration via somatic embryogenesis has not beenapplied in Arabidopsis so far. Recently, it was found that development ofArabidopsis somatic embryos can be induced in the culture of immature zygotic embryos and that the callus phase is not nocessary for the initiation of embryogenesis. The aim of the presented research was to determinate the in vitro cultureconditions enabling high efficiency of somatic embryo induction and theirconversion into plants. The influence of induction medium composition including liquid or agar medium, type and concentration of auxin, carbohydrates andammonium sources as well as duration of auxin treatment of explants on DSEefficiency were evaluated. Advantages of described regeneration system via DSEare as follows: short time needed to induce somatic embryos (10-15 days), highefficiency ofthe process (more than 80% explants responded), numerous embryos produced per explant (on average 17) and high percentage of embryo conversion into fertile plants (70-80%).

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

90

End page:

98

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139269 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open