Subtitle:

Growth kinetics of suspension culture of wheat (Triticum aestivum L.)influenced by sucrose concentration in the medium

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The suspension culture was initiated from callus derived from immature inflorescences of winter wheat var Almari. The aim of our investigation was evaluation of the influence of twice increased sucrose concentration in the mediumon the physicochemical properties and cell morphology during 15 days of suspension culture. The number of cells, volume, weight, shape and viability weremonitored in a few day intervals. Moreover, pH, conductivity and osmolality ofmedium were measured. The suspensions that were maintained in media containing 30 and 60 g of sucrose per dm^ were characterised by a similar cell viability and growth kinetics tendency. However, from the day of culture, increased concentration ofsucrose inhibited the cell proliferation rate. This effectwas associated with decreased volume and dry weight of cells, which indicatesthat sucrose influences the production of cell biomass. The morphological character of cells was independent of the sucrose concentration, the cell area andcircularity changed similarly for both media. The area was increased and circularity decreased. Increased concentration of sucrose resulted in lowered pH ofthe medium during the whole culture period. There was no statistically significant influence of sucrose on the conductivity and osmolality changes in media.The results suggest that higher level ofsucrose in the medium can be disadvantageous for growth kinetics ofwheat suspension. It was not connected with medium osmotic potential changes or cell-medium ion exchange possibilities.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

104

End page:

110

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139274 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open