Subtitle:

Early stages of embryogenesis after fertilization in in vitro condition

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

Ovules of plum cultivars (Sweet Common Prune, Herman, Gilbert, SanctusHubertus, Cacanska Rodna, Ćaćanska Najbolia) were pollinated with pollen ofcv. Stanley on the White medium with addition of 15% sucrose. The nucellusesexcised from the fertilized ovules were cultivated on Norstog medium with addition of 3,4% sucrose. The process of fertilization after pollination in the in vitro condition was determined on the basis of the paraffin cross-sections: thedegeneration of synergid after entering the pollen tube, the fertilization of eggcell were confirmed. The microscopic observations showed the swelling ofmicropylar part of nucellus. The paraffin cross-sections showed a many-layerkalotka (peculiar morphological structure) and swelled cells of nucellus amongwhich the embryo sac was observed. The presence of embryo without endosperm or the presence of endosperm without embryo in the embryo sac wasnoted.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

54

End page:

56

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139250 ; IChB B-49

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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