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Tyburski, Jarosław ; Studzińska, Anna ; Daca, Patrycja ; Tretyn, Andrzej
PCR in real time. The methods of data analysis
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The accuracy of real-time PCR (RT-PCR) experiment is strongly dependent on the mathematical models of data analysis, on which the quantitative methods are based. In this review, we discuss the key steps of analysing data from real-time PCR experiments. These are the treshold cycle determination, estimation of real-time PCR amplification efficiency and amplicon quantification. The fit point method and the second derivative method are commonly used to determine a treshold cycle value, which is a cycle number in the early exponential phase of PCR that is used to calculate the initial amount of template DNA.
Biotechnologia, vol.80, 1 (2008)-.
0860-7796 ; oai:rcin.org.pl:74139
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
2 lip 2019
19069
https://rcin.org.pl./publication/97118
Nazwa wydania | Data |
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PCR w czasie rzeczywistym. Metody analizy danych | 2 paź 2020 |
Studzińska, Anna Tyburski, Jarosław Daca, Patrycja Tretyn, Andrzej
Sławińska, Anna Siwek, Maria
Tyczewska, Agata Bąkowska- Żywicka, Kamila
Pieczyński, Marcin Bielewicz, Dawid Dolata, Jakub Szweykowska-Kulińska, Zofia