Subtitle:

PCR in real time. The methods of data analysis

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

The accuracy of real-time PCR (RT-PCR) experiment is strongly dependent on the mathematical models of data analysis, on which the quantitative methods are based. In this review, we discuss the key steps of analysing data from real-time PCR experiments. These are the treshold cycle determination, estimation of real-time PCR amplification efficiency and amplicon quantification. The fit point method and the second derivative method are commonly used to determine a treshold cycle value, which is a cycle number in the early exponential phase of PCR that is used to calculate the initial amount of template DNA.

Relation:

Biotechnologia, vol.80, 1 (2008)-.

Volume:

80

Issue:

1

Start page:

86

End page:

96

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:74139

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open