Subtitle:

L-lysine biosynthesis

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Amino acid fermentation has grown into a global industry. Market development has been particularly dynamic for the flavor-enhancer glutamate and animal feed amino acids: L-lysine, DL-methionine, L-threonine, and L-tryptophan. These amino acids are manufactured using high performance Corynebacterium glutamicum. Production strains have been traditionally constructed by repeating random mutation and selection. This classical method has generated a variety of mutation, such as auxotrophs, analog-resistant mutants and transport mutants. The complete genome sequence of the wild-type strain of C. glutamicum has been established and analysed to improve the understending of the molecular biology and physiology of this organism. A novel methodology that merges genomics with classical strains improvement has been developed and applied for the reconstruction of classicaly derived production strains. In addition, modern technologies such as metabolic flux analysis and metabolic control analysis have enabled quantification of carbon fluxes. The fundamental information obtained has been the basis for further strain improvement.

Relation:

Biotechnologia, vol.80, 1 (2008)-.

Volume:

80

Issue:

1

Start page:

53

End page:

70

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:74059

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open