• Search in all Repository
  • Literature and maps
  • Archeology
  • Mills database
  • Natural sciences

Search in Repository

How to search...

Advanced search

Search in Literature and maps

How to search...

Advanced search

Search in Archeology

How to search...

Advanced search

Search in Mills database

How to search...

Advanced search

Search in Natural sciences

How to search...

Advanced search

RCIN and OZwRCIN projects

Object

Title: Puriflcation and properties of chitin deacetylase from Absidiaorchidis

Creator:

Szewczyk, Krzysztof W. ; Orzeszak, Małgorzata

Date issued/created:

2002

Resource type:

Text

Subtitle:

Puriflcation and properties of chitin deacetylase from Absidiaorchidis

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Methods of purification of chitin deacetylase are discussed. A two stepmethod of purification of chitin deacetylase from mycelial extracts ofthe fungusAbsidia orchidis by chromatography is presented. The crude enzyme extract waspurified by a gel chromatography and then by ion exchange chromatography.Specific activity of purified enzyme was 12.3 U/mg and final purification degreewas 147. The apparent molecular mass of the enzyme was 75 kDa. When 0 -hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pHfor enzyme activity was 5,5 and the optimum temperature was 50°C

Relation:

Biotechnologia, vol.57, 2 (2002)-.

Volume:

57

Issue:

2

Start page:

48

End page:

59

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138077 ; IChB B-53

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open

Object collections:

Last modified:

Oct 2, 2020

In our library since:

Sep 7, 2020

Number of object content downloads / hits:

176

All available object's versions:

https://rcin.org.pl./publication/173239

Show description in RDF format:

RDF

Show description in RDFa format:

RDFa

Show description in OAI-PMH format:

OAI-PMH

×

Citation

Citation style:

This page uses 'cookies'. More information