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Puriflcation and properties of chitin deacetylase from Absidiaorchidis
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Methods of purification of chitin deacetylase are discussed. A two stepmethod of purification of chitin deacetylase from mycelial extracts ofthe fungusAbsidia orchidis by chromatography is presented. The crude enzyme extract waspurified by a gel chromatography and then by ion exchange chromatography.Specific activity of purified enzyme was 12.3 U/mg and final purification degreewas 147. The apparent molecular mass of the enzyme was 75 kDa. When 0 -hydroxyethylated chitin (glycol chitin) was used as a substrate, the optimum pHfor enzyme activity was 5,5 and the optimum temperature was 50°C
Biotechnologia, vol.57, 2 (2002)-.
0860-7796 ; oai:rcin.org.pl:138077 ; IChB B-53
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 7, 2020
174
https://rcin.org.pl./publication/173239
Edition name | Date |
---|---|
Oczyszczanie i właściwości deacetylazy chityny z Absidia orchidis | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Szewczyk, Krzysztof W.
Szewczyk, Krzysztof W.