Podwyszyńska, Małgorzata ; Kuras, Anita ; Korbin, Małgorzata
ISSR evaluation of genetic stability of Polish tulip cultivars propagated in vitro
Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS
Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN
The aim of the study was an early detection of somaclonal variation (SV) which could occur within the micropropagated plant material. Shoot cultures of the Polish cultivars were used. Five cultivars derived from breeder A and four from breeder B. They were propagated in vitro for one or two years. The molecular markers (inter-simple sequence repeat, ISSR) were utilized for analysis of putative DNA polymorphism between standard plants (propagated traditionally) belonging to tested cultivars and somaclonal variants derived from them. Within the nine studied genotypes, for five of them, the ISSR analysis performed with ten primers did not reveal polymorphism between standard and micropropagated plants. The analysis of the other four cultivars, all derived from breeder B, showed that some of the plants, micropropagated either for one or two years, differed slightly from standard. Basing on ISSR data, the L'PGMA dendrogram showing genetic similarity of the analysed plants was generated and clusters grouping cultivars, their standards and micropropagated plants were noted.
Biotechnologia, vol.89, 2 (2010)-.
oai:rcin.org.pl:71598 ; IChB B-84
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk
2 paź 2020
27 maj 2019
369
https://rcin.org.pl./publication/93858
Nazwa wydania | Data |
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Ocena stabilności genetycznej rozmnażanych in vitro polskich odmian tulipanów przy użyciu markerów molekularnych ISSR | 2 paź 2020 |
Kuras, Anita Korbin, Małgorzata Żurawicz, Edward
Podwyszyńska. Małgorzata Gabryszewska, Eleonora Korbin, Małgorzata Jasiński, Artur
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Korbin, Małgorzata
Nowak, Jacek K.