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Topophysis in adventitious shoots regeneration in vitro in chrysanthemum
Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS
Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN
Success of breeding programmes using adventitious shoots technique often depends on the regeneration efficiency. The aim of this study was to investigate the influence of topophysical position of explants on the efficiency of adventitious shoots regeneratio. Uniform, single shoots of chrysanthemum ‘Satinbleu’ propagated in vitro were divided equally into three topophysical zones: distal, central and proximal. Two leaves and two internodes were isolated from each zone. The explants were cultured for 12 weeks on MS medium supplemented with 0.6 mg 1 ' BAP and 2.0 mg I ' 1AA. Duo to the highest adventitious shoots regeneration capability and the earliest formation of the shoots, the most suitable explants for breeding programmes for chrysanthemum are internodes excised from distal part of in vitro plantlet.
Biotechnologia, vol.89, 2 (2010)-.
oai:rcin.org.pl:71588 ; IChB B-84
Biblioteka Instytutu Chemii Bioorganicznej PAN
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
May 27, 2019
824
https://rcin.org.pl./publication/93839
Edition name | Date |
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Zjawisko topofizy w regeneracji pędów przybyszowych in vitro u chryzantemy wielkokwiatowej | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Zalewska, Małgorzata Lema-Rumińska, Justyna