Subtitle:

The control and eradication of bacterial infections and contaminations in plant tissue culture

Contributor:

Institute of Bioorganic Chemistry PAS ; Committee on Biotechnology PAS

Publisher:

Instytut Chemii Bioorganicznej PAN ; Komitet Biotechnologii PAN

Description:

prace przeglądowe

Abstract:

Bacterial contamination is a serious problem in plant tissue culture. In in vitro cultures, bacteria are introduced, with the initial which is most frequently explants, but bacterial contamination can also come from the laboratory environment or from the staff themselves. Exogenous bacteria are easier to deal with, but endogenous bacteria remain problematic. Standard sterilization with ethanol, NaOCl or HgCl2 and with antibiotics can now be enriched with new components (NaDCC, AgN03, nano-silver) or sanitation products (PPM™, ProClin® 300, Biosept 33 SL, Vitrofural®, Dekaben). Some of these can be incorporated into a multiplication and rooting medium for one or more passages, if they are not phytotoxic to the plant explants. A special problem is presented by cryptic or viable but not cultivable bacteria which can be unable to multiply during many passages, but finally be disclosed in mass. The issue is, therefore, to Find and apply tools for detection of selective media and/or molecular markers. The above questions are discussed in the present paper based on the literature and results of our own study.

Relation:

Biotechnologia, vol.89, 2 (2010)-.

Volume:

89

Issue:

2

Start page:

57

End page:

71

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

oai:rcin.org.pl:71580 ; IChB B-84

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open