Subtitle:

The importance of precultures for micropropagation of explants containing endophytes

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

An endophyte is a microorganism that spends most of its life cycle interor intra-cellularily of the host organism without causing its disease. As a resultof the high frequency of endophytes in plants, it is virtually impossible to isolate tissue or cell explants free from contaminants. Consequently, preculture(i.e. “stage 0”) is necessary before disinfecting and stabilisation of in vitro cultures, to eliminate explants that are sources of persistent contamination. Particularly dangerous are latent bacterial contaminants, which initially do not causeany symptoms and are propagated with plant material. Their presence becomesconspicuous after the passage to a sucrose-free medium or after transplantationto the soil.

Relation:

Biotechnologia, vol.55, 4 (2001)-.

Volume:

55

Issue:

4

Start page:

87

End page:

99

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138591 ; IChB B-50

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open