Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
The article briefly presents the design and chemical synthesis of parallel stranded DNA withformation of reverse Watson-Crick base pairs. Tchurikov et al. attempted an analysis of parallelRNA-RNA duplex formation. Single stranded RNA, prepared by in vitro transcription with T7polymerase using and ^H nucleoside 5’-triphosphates, were appropriately hybridized andanalysed by polyacrylamide gel electrophoresis prior and after RNase A digestion. Results obtained allow to conclude on the formation of parallel stranded, RNA stable to RNase A. Thepresented data show the importance of intramolecular parallel association of the single strandedRNA during its folding.
Biotechnologia, vol.37, 2 (1997)-.
0860-7796 ; oai:rcin.org.pl:146113
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Nov 18, 2020
Nov 5, 2020
98
https://rcin.org.pl./publication/181578
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Nowak, Jacek K.