Subtitle:

Application of callus tissue in the studies on molecular mechanism offlower induction

Publisher:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstract:

Flowering is a crucial turning point in the life cycle of most plants. The process offlowering is controlled by external factors such as light and temperature.Floral induction is the first step in the transition from the vegetative to reproductive stage of development. In the photoperiodically sensitive plants this process is regulated by the duration of light and darkness during a 24-h cycle. Theaim of our study was to determine whether the undifferentiated callus tissueobtained from cotyledons, is suitable for molecular investigations on the mechanisms of flower induction. The callus tissue was obtained from cotyledons ofPharbitis nil plants, which were cultivated in inductive or non-inductive conditions. The callus obtained after two subcultures was used for isolation of RNA.The total RNA was extracted as described by Chomczynski (1993). We have examined the changes in the pattern of RNA in these two types of callus, using thetechnique of differential display by the polymerase chain reaction (PCR). Differential display is a method for the identification and cloning of differentiallyexpressed eucaryotic genes.

Relation:

Biotechnologia, vol.53, 2 (2001)-.

Volume:

53

Issue:

2

Start page:

165

End page:

169

Detailed Resource Type:

Article

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:139325 ; IChB B-49

Source:

Library of Institute of Bioorganic Chemistry PAS

Language:

pol

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Creative Commons Attribution BY-SA 4.0 license

Terms of use:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitizing institution:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Original in:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Projects co-financed by:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Access:

Open