Trzmiel, Tadeusz ; Szczęsna-Antczak, Mirosława ; Rzyska, Małgorzata
Subtilisin embedded in TAC fibre - properties and application
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The embodiment ofsubtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose(TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/gwas obtained with 16.6% of yield. The properties ofsubtilisin embedded in TACfibre were investigated. The preparation showed optimal proteolytic activity atpH 9 and temperature 65-70°C. It was stable in a wider range of pH and temperature than the native subtilisin. The value for haemoglobin was 2.6 • lO'^ M.The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied forhydrolysis of casein.
Biotechnologia, vol.55, 4 (2001)-.
0860-7796 ; oai:rcin.org.pl:138631 ; IChB B-50
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 11, 2020
328
https://rcin.org.pl./publication/173975
Edition name | Date |
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Subtilizyna osadzona na włóknach triacetylocelulozowych - właściwości i zastosowanie | Oct 2, 2020 |
Antczak, Tadeusz Szczęsna-Antczak, Mirosława
Antczak, Tadeusz Szczęsna-Antczak, Mirosława
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Trzmiel, Tadeusz