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Subtilisin embedded in TAC fibre - properties and application
Subtitle:Subtilisin embedded in TAC fibre - properties and application
Creator:Trzmiel, Tadeusz ; Szczęsna-Antczak, Mirosława ; Rzyska, Małgorzata
Publisher:Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
Date issued/created: Subject and Keywords: Abstract:The embodiment ofsubtilisin from Bacillus subtilis IBTC-3 in triacetylocellulose(TAC) fibre was studied. The fibre preparation of proteolytic activity 51.3 AmU/gwas obtained with 16.6% of yield. The properties ofsubtilisin embedded in TACfibre were investigated. The preparation showed optimal proteolytic activity atpH 9 and temperature 65-70°C. It was stable in a wider range of pH and temperature than the native subtilisin. The value for haemoglobin was 2.6 • lO'^ M.The TAC-fibre biocatalyst, in the fixed bed column, was successfully applied forhydrolysis of casein.
Relation:Biotechnologia, vol.55, 4 (2001)-.
Volume: Issue: Start page: End page: Resource type: Detailed Resource Type: Format: Resource Identifier: Source:Library of Institute of Bioorganic Chemistry PAS
Language: Language of abstract: Temporal coverage: Rights:Creative Commons Attribution BY-SA 4.0 license
Terms of use: Digitizing institution:Institute of Bioorganic Chemistry of the Polish Academy of Science
Original in:Institute of Bioorganic Chemistry of the Polish Academy of Science
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