Inny tytuł:

Development of an automated yeast identification system by the application of PCR technique: a comparison of Inter-LINE PCR with reference methods

Wydawca:

Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS

Abstrakt:

In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, whichoriginated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LlNE-sequences. These were usedfor the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera. The IL-PCR technique using the above primers is describedand compared to reference methods such as Arbitrarily Primed-PCR and PulseField Gel Electrophoresis (PFGE).A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fmgerprints. A database for an automated yeast identification system is proposed.

Czasopismo/Seria/cykl:

Biotechnologia, vol.55, 4 (2001)-.

Tom:

55

Zeszyt:

4

Strona pocz.:

122

Strona końc.:

134

Szczegółowy typ zasobu:

Article

Format:

application/pdf

Identyfikator zasobu:

0860-7796 ; oai:rcin.org.pl:138601 ; IChB B-50

Źródło:

Library of Institute of Bioorganic Chemistry PAS

Język:

pol

Język streszczenia:

eng

Zakres czasowy:

1988-2010

Prawa:

Creative Commons Attribution BY-SA 4.0 license

Zasady wykorzystania:

Copyright-protected material. [CC BY-SA 4.0] May be used within the scope specified in Creative Commons Attribution BY-SA 4.0 license, full text available at:

Digitalizacja:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Lokalizacja oryginału:

Institute of Bioorganic Chemistry of the Polish Academy of Science

Dofinansowane ze środków:

Operational Program Digital Poland, 2014-2020, Measure 2.3: Digital accessibility and usefulness of public sector information; funds from the European Regional Development Fund and national co-financing from the state budget.

Dostęp:

Open