The importance of precultures for micropropagation of explants containing endophytes
Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
An endophyte is a microorganism that spends most of its life cycle interor intra-cellularily of the host organism without causing its disease. As a resultof the high frequency of endophytes in plants, it is virtually impossible to isolate tissue or cell explants free from contaminants. Consequently, preculture(i.e. “stage 0”) is necessary before disinfecting and stabilisation of in vitro cultures, to eliminate explants that are sources of persistent contamination. Particularly dangerous are latent bacterial contaminants, which initially do not causeany symptoms and are propagated with plant material. Their presence becomesconspicuous after the passage to a sucrose-free medium or after transplantationto the soil.
Biotechnologia, vol.55, 4 (2001)-.
0860-7796 ; oai:rcin.org.pl:138591 ; IChB B-50
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
2 paź 2020
11 wrz 2020
695
https://rcin.org.pl./publication/173925
Nazwa wydania | Data |
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Znaczenie prekultur w mikrorozmnażaniu roślin zasiedlonych przez endofity | 2 paź 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Orlikowska, Teresa Sobiczewski, P. Zawadzka, M.
Zenkteler, Elżbieta
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