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The importance of precultures for micropropagation of explants containing endophytes
Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN
An endophyte is a microorganism that spends most of its life cycle interor intra-cellularily of the host organism without causing its disease. As a resultof the high frequency of endophytes in plants, it is virtually impossible to isolate tissue or cell explants free from contaminants. Consequently, preculture(i.e. “stage 0”) is necessary before disinfecting and stabilisation of in vitro cultures, to eliminate explants that are sources of persistent contamination. Particularly dangerous are latent bacterial contaminants, which initially do not causeany symptoms and are propagated with plant material. Their presence becomesconspicuous after the passage to a sucrose-free medium or after transplantationto the soil.
Biotechnologia, vol.55, 4 (2001)-.
0860-7796 ; oai:rcin.org.pl:138591 ; IChB B-50
Biblioteka Instytutu Chemii Bioorganicznej PAN
Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Instytut Chemii Bioorganicznej Polskiej Akademii Nauk
Oct 2, 2020
Sep 11, 2020
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https://rcin.org.pl./publication/173925
Edition name | Date |
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Znaczenie prekultur w mikrorozmnażaniu roślin zasiedlonych przez endofity | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Orlikowska, Teresa Sobiczewski, P. Zawadzka, M.
Zenkteler, Elżbieta
Dastych, Wojciech Zenkteler, Elżbieta
Nowak, Jacek K.