RCIN and OZwRCIN projects

Object

Title: Classic techniques for improvement of industrial yeast strains: Part III - A method for enucleation of Saccharomyces sp.

Creator:

Salek, Anna Teresa

Date issued/created:

2002

Resource type:

Tekst

Subtitle:

Classic techniques for improvement of industrial yeast strains: Part III - A method for enucleation of Saccharomyces sp.

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Abstract:

The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficientmutants of Saccharomyces cerevisiae were enucleated after treatment withcytochalasin B.Removal of nuclei from protruding sphaeroplasts was induced bycentrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCU and ATP (10 pM) in the enucleation medium prevented theformation of fragile products or aggregation. Cytoplasts could be stored for atleast 1 day without visible deterioration.

Relation:

Biotechnologia, vol.56, 1 (2002)-.

Volume:

56

Issue:

1

Start page:

187

End page:

194

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:138414 ; IChB B-52

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

eng

Language of abstract:

eng

Temporal coverage:

1988-2010

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganicznej Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

Otwarty

Object collections:

Last modified:

Oct 2, 2020

In our library since:

Sep 10, 2020

Number of object content downloads / hits:

59

All available object's versions:

https://rcin.org.pl./publication/173779

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