Committee on Biotechnology PAS ; Institute of Bioorganic Chemistry PAS
The first method for enucleation of yeast Saccharomyces cerevisiae is reported. Various strains, including some killer strain and respiratory-deficientmutants of Saccharomyces cerevisiae were enucleated after treatment withcytochalasin B.Removal of nuclei from protruding sphaeroplasts was induced bycentrifugation in a Percoll density gradient. The enucleation yield (which averaged about 80%) and the quality of the cytoplasts were best when the yeast culture had been synchronized with nocodazole before the preparation. The presence of 1 mM CaCU and ATP (10 pM) in the enucleation medium prevented theformation of fragile products or aggregation. Cytoplasts could be stored for atleast 1 day without visible deterioration.
Biotechnologia, vol.56, 1 (2002)-.
0860-7796 ; oai:rcin.org.pl:138414 ; IChB B-52
Library of Institute of Bioorganic Chemistry PAS
Creative Commons Attribution BY-SA 4.0 license
Institute of Bioorganic Chemistry of the Polish Academy of Science
Institute of Bioorganic Chemistry of the Polish Academy of Science
Oct 2, 2020
Sep 10, 2020
66
https://rcin.org.pl./publication/173779
Edition name | Date |
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Classic techniques for improvement of industrial yeast strains: Part III - A method for enucleation of Saccharomyces sp. | Oct 2, 2020 |
Borkowska, Bożenna
Ziółkowski, Piotr Babula- Skowrońska, Danuta Kaczmarek, Małgorzata Cieśla, Agata Sadowski, Jan
Salek, Anna Teresa
Salek, Anna Teresa
Salek, Anna Teresa
Salek, Anna Teresa