Subtitle:

Suitability of Thermus ruber as a source of trehalose synthase

Publisher:

Komitet Biotechnologii PAN ; Instytut Chemii Bioorganicznej PAN

Description:

prace eksperymentalne

Abstract:

Thermus ruber is a producer of trehalose synthase, which catalyses the conversion of maltose into trehalose by intramolecular transglucosylation. The specific activity of cell-free extract of Thermus ruber cultivated on a media without saccharides was 0.016-0.028 U/mg protein and it increased up to 0.086 U/mg in the presence of 0.5?^ maltose in the culture broth. The maximum degree of maltose conversion of about 90“6 was achieved at 10% substrate concentration. The trehalose synthase does not catalyse formation of trehalose from malto- tetraose, maltohexaose and other oligosaccharides. The optimal temperature for enzyme activity was 65°C. A maximum activity of the maltose transglucosylation was performed at pH 6.5. The highest yield of trehalose synthase was attained during cultivation of bacteria at 55°C for 48 h on media composed of 0.5% peptone, 0.1% yeast extract and 0.5% maltose or starch.

Relation:

Biotechnologia, vol.80, 1 (2008)-.

Volume:

80

Issue:

1

Start page:

168

End page:

176

Detailed Resource Type:

Artykuł

Format:

application/pdf

Resource Identifier:

0860-7796 ; oai:rcin.org.pl:74272

Source:

Biblioteka Instytutu Chemii Bioorganicznej PAN

Language:

pol

Language of abstract:

eng

Temporal coverage:

2008

Rights:

Licencja Creative Commons Uznanie autorstwa-Na tych samych warunkach 4.0

Terms of use:

-

Digitizing institution:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Original in:

Instytut Chemii Bioorganiczneji Polskiej Akademii Nauk

Projects co-financed by:

Program Operacyjny Polska Cyfrowa, lata 2014-2020, Działanie 2.3 : Cyfrowa dostępność i użyteczność sektora publicznego; środki z Europejskiego Funduszu Rozwoju Regionalnego oraz współfinansowania krajowego z budżetu państwa

Access:

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